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Quantitation of pulmonary fungal burden in Paracoccidioides brasiliensis-infected mice by real-time PCR
Although colony-forming unit (CFU) counting is widely used to quantify fungal load in tissue from animal experimentally infected with Paracoccidioides brasiliensis, several technical disadvantages have been described. Here we developed highly accurate quantitative PCR (qPCR) assays to determine the...
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| Publié dans: | Rev Inst Med Trop Sao Paulo |
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| Auteurs principaux: | , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Instituto de Medicina Tropical
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6300791/ https://ncbi.nlm.nih.gov/pubmed/30570075 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1678-9946201961002 |
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