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Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics

Despite rapid developments in single cell sequencing, sample-specific batch effects, detection of cell multiplets, and experimental costs remain outstanding challenges. Here, we introduce Cell Hashing, where oligo-tagged antibodies against ubiquitously expressed surface proteins uniquely label cells...

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Detalles Bibliográficos
Publicado en:Genome Biol
Main Authors: Stoeckius, Marlon, Zheng, Shiwei, Houck-Loomis, Brian, Hao, Stephanie, Yeung, Bertrand Z., Mauck, William M., Smibert, Peter, Satija, Rahul
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2018
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6300015/
https://ncbi.nlm.nih.gov/pubmed/30567574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-018-1603-1
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