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Isolation and short term culture of primary keratinocytes, hair follicle populations, and dermal cells from newborn mice and keratinocytes from adult mice, for in vitro analysis and for grafting to immunodeficient mice.

Protocols for preparing and culturing primary keratinocytes from newborn and adult mouse epidermis have evolved over the last 35 years. The present protocol is now routinely applied to mice of various genetic backgrounds for in vitro studies of signaling pathways in differentiation and in cell trans...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Nat Protoc
Hauptverfasser: Lichti, Ulrike, Anders, Joanna, Yuspa, Stuart H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6299324/
https://ncbi.nlm.nih.gov/pubmed/18451788
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.50
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