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A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)

BACKGROUND: Genome editing of monocot plants can be accomplished by using the components of the CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR associated Cas9) technology specifically optimized for these types of plants. Here, we present the development of RNA-guided Ca...

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Detalhes bibliográficos
Publicado no:Plant Methods
Main Authors: Gasparis, Sebastian, Kała, Maciej, Przyborowski, Mateusz, Łyżnik, Leszek A., Orczyk, Wacław, Nadolska-Orczyk, Anna
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6297969/
https://ncbi.nlm.nih.gov/pubmed/30568723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-018-0382-8
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