Construction of ovine GH-pmKate2N expression vector and its uptake by ovine spermatozoa using different methods

This study aims to produce transgenic ovine spermatozoa bearing Ossimi sheep growth hormone (Os_GH) cDNA using different methods. The complete coding sequence of Os_GH has been registered in GenBank accession no. KP221575. The sequence of Os_GH cDNA has been subcloned into pmkate2-N expression vecto...

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Detalhes bibliográficos
Publicado no:J Genet Eng Biotechnol
Main Authors: Shakweer, W.M.E., Hafez, Y.M., El-Sayed, A.A., Awadalla, I.M., Mohamed, M.I.
Formato: Artigo
Idioma:Inglês
Publicado em: Academy of Scientific Research and Technology, Egypt 2017
Assuntos:
I : Animal Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6296570/
https://ncbi.nlm.nih.gov/pubmed/30647637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jgeb.2017.04.001
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