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Development of a highly sensitive enzyme-linked immunosorbent assay (ELISA) through use of poly-protein G-expressing cell-based microplates

The sensitivity of traditional enzyme-linked immunosorbent assays (ELISAs) is limited by the low binding avidity and heterogeneous orientation of capture antibodies coated on polystyrene-based microplates. Here, we developed a highly sensitive ELISA strategy by fixing poly-protein G-expressing cells...

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Dettagli Bibliografici
Pubblicato in:Sci Rep
Autori principali: Chen, Yi-Jou, Chen, Michael, Hsieh, Yuan-Chin, Su, Yu-Cheng, Wang, Chang-Hung, Cheng, Chiu-Min, Kao, An-Pei, Wang, Kai-Hung, Cheng, Jing-Jy, Chuang, Kuo-Hsiang
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2018
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6294806/
https://ncbi.nlm.nih.gov/pubmed/30552393
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-36192-8
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