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Photobleaching Kinetics and Time-Integrated Emission of Fluorescent Probes in Cellular Membranes

Since the pioneering work of Hirschfeld, it is known that time-integrated emission (TiEm) of a fluorophore is independent of fluorescence quantum yield and illumination intensity. Practical implementation of this important result for determining exact probe distribution in living cells is often hamp...

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Publicat a:Molecules
Autors principals: Wüstner, Daniel, Christensen, Tanja, Solanko, Lukasz M., Sage, Daniel
Format: Artigo
Idioma:Inglês
Publicat: MDPI 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6271172/
https://ncbi.nlm.nih.gov/pubmed/25076144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules190811096
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