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Recombinant Expressed Vector pET32a (+) S Constructed by Ligation Independent Cloning

The aim of this work was to develop a new method for constructing vectors, named ligation-independent cloning (LIC) method. We constructed the S label expression vector and recombinant pET32a (+) S-phoN2 by LIC. The recombinant proteins were expressed in E. coli at a high level, and then the specifi...

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Détails bibliographiques
Publié dans:Molecules
Auteurs principaux: Wang, Yu, Gong, Guo-Hua, Wei, Cheng-Xi, Liang, Long, Zhang, Bin
Format: Artigo
Langue:Inglês
Publié: MDPI 2014
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6271046/
https://ncbi.nlm.nih.gov/pubmed/25310147
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules191016179
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