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Recombinant Expressed Vector pET32a (+) S Constructed by Ligation Independent Cloning
The aim of this work was to develop a new method for constructing vectors, named ligation-independent cloning (LIC) method. We constructed the S label expression vector and recombinant pET32a (+) S-phoN2 by LIC. The recombinant proteins were expressed in E. coli at a high level, and then the specifi...
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| Publicado no: | Molecules |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MDPI
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6271046/ https://ncbi.nlm.nih.gov/pubmed/25310147 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules191016179 |
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