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Isolation of photoreceptors from mature, developing, and regenerated zebrafish retinas, and of microglia/macrophages from regenerating zebrafish retinas

This paper describes experimental procedures for the dissociation of retinal cells of the zebrafish (Danio rerio) for subsequent fluorescence-activated cell sorting (FACS) and gene expression studies. Methods for dissociation of zebrafish retinas followed by FACS, and RNA isolation, were optimized....

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Publicat a:Exp Eye Res
Autors principals: Sun, Chi, Mitchell, Diana M., Stenkamp, Deborah L.
Format: Artigo
Idioma:Inglês
Publicat: 2018
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6261699/
https://ncbi.nlm.nih.gov/pubmed/30096325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.exer.2018.08.002
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