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Floxed-Cassette Allelic Exchange Mutagenesis Enables Markerless Gene Deletion in Chlamydia trachomatis and Can Reverse Cassette-Induced Polar Effects

As obligate intracellular bacteria, Chlamydia spp. have evolved numerous, likely intricate, mechanisms to create and maintain a privileged intracellular niche. Recent progress in elucidating and characterizing these processes has been bolstered by the development of techniques enabling basic genetic...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Main Authors: Keb, G., Hayman, R., Fields, K. A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6256029/
https://ncbi.nlm.nih.gov/pubmed/30224436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.00479-18
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