Improving redox sensitivity of roGFP1 by incorporation of selenocysteine at position 147

OBJECTIVE: Redox-sensitive green fluorescent protein (roGFP) is a genetically-encoded redox-sensitive protein used to detect cellular oxidative stress associated with reactive oxygen species production. Here we replaced the cysteine at position 147 of roGFP1 (variant of roGFP) with selenocysteine in...

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Publicado en:BMC Res Notes
Autores principales: Stanford, Katherine R., Ajmo, Joanne M., Bahia, Parmvir K., Hadley, Stephen H., Taylor-Clark, Thomas E.
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2018
Materias:
Research Note
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6249920/
https://ncbi.nlm.nih.gov/pubmed/30466490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-018-3929-x
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