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Tracking yeast pheromone receptor Ste2 endocytosis using fluorogen-activating protein tagging
To observe internalization of the yeast pheromone receptor Ste2 by fluorescence microscopy in live cells in real time, we visualized only those molecules present at the cell surface at the time of agonist engagement (rather than the total cellular pool) by tagging this receptor at its N-terminus wit...
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| Publicado no: | Mol Biol Cell |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The American Society for Cell Biology
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6249837/ https://ncbi.nlm.nih.gov/pubmed/30207829 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E18-07-0424 |
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