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Tracking yeast pheromone receptor Ste2 endocytosis using fluorogen-activating protein tagging

To observe internalization of the yeast pheromone receptor Ste2 by fluorescence microscopy in live cells in real time, we visualized only those molecules present at the cell surface at the time of agonist engagement (rather than the total cellular pool) by tagging this receptor at its N-terminus wit...

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Bibliografski detalji
Izdano u:Mol Biol Cell
Glavni autori: Emmerstorfer-Augustin, Anita, Augustin, Christoph M., Shams, Shadi, Thorner, Jeremy
Format: Artigo
Jezik:Inglês
Izdano: The American Society for Cell Biology 2018
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6249837/
https://ncbi.nlm.nih.gov/pubmed/30207829
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E18-07-0424
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