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Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay

Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus, which belongs to the family Coronaviridae. It causes watery diarrhea, vomiting, and dehydration in newborn piglets. A sensitive RT-PCR method is urgently required to detect PDCoV infection. In this study, we developed and evaluated...

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Detalhes bibliográficos
Publicado no:Biomed Res Int
Main Authors: Ma, Lei, Zeng, Fanwen, Huang, Bihong, Cong, Feng, Huang, Ren, Ma, Jingyun, Guo, Pengju
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6247729/
https://ncbi.nlm.nih.gov/pubmed/30533434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2018/5035139
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