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Efficient Counterselection for Methylococcus capsulatus (Bath) by Using a Mutated pheS Gene

Methylococcus capsulatus (Bath) is a representative gammaproteobacterial methanotroph that has been studied extensively in diverse research fields. The sacB gene, which encodes levansucrase, causing cell death in the presence of sucrose, is widely used as a counterselectable marker for disruption of...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Ishikawa, Masahito, Yokoe, Sho, Kato, Souichiro, Hori, Katsutoshi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6238059/
https://ncbi.nlm.nih.gov/pubmed/30266726
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01875-18
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