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On the damage done to the structure of the Thermoplasma acidophilum proteasome by electron radiation

It has long been known that proteins are damaged when they are exposed to the electron beam in an electron microscope. Here we show that exposure to electrons under cryo‐EM conditions leads to a small change in the quaternary structure of the Thermoplasma acidophilum proteasome, and that backbones a...

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Publicat a:Protein Sci
Autors principals: Wang, Jimin, Liu, Zheng, Crabtree, Robert H., Frank, Joachim, Moore, Peter B.
Format: Artigo
Idioma:Inglês
Publicat: John Wiley & Sons, Inc. 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6237698/
https://ncbi.nlm.nih.gov/pubmed/30242932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3511
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