Purification and Characterization of Glutathione Binding Protein GsiB from Escherichia coli

OBJECTIVES: To purify and characterize the glutathione binding protein GsiB of glutathione importer (GSI) in Escherichia coli (E. coli). RESULTS: The coding sequence of GsiB was cloned from E. coli MG1655 and expressed in BL21(DE3). GsiB protein was expressed and purified to homogeneity using Ni-aff...

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Detalhes bibliográficos
Publicado no:Biomed Res Int
Main Authors: Wang, Zhongshan, Xia, Xiaokun, Zhang, Meixian, Fang, Jiawei, Li, Yanqiang, Zhang, Meng
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi 2018
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6236770/
https://ncbi.nlm.nih.gov/pubmed/30515393
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2018/3429569
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