Gene Expression Analysis of Endothelial Cells Exposed to Shear Stress Using Multiple Parallel-plate Flow Chambers

We describe a workflow for the analysis of gene expression from endothelial cells subject to a steady laminar flow using multiple monitored parallel-plate flow chambers. Endothelial cells form the inner cellular lining of blood vessels and are chronically exposed to the frictional force of blood flo...

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Bibliografske podrobnosti
izdano v:J Vis Exp
Main Authors: Man, H.S. Jeffrey, Sukumar, Aravin N., Ku, Kyung Ha, Dubinsky, Michelle K., Subramaniam, Noeline, Marsden, Philip A.
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2018
Teme:
Bioengineering
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235583/
https://ncbi.nlm.nih.gov/pubmed/30394398
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58478
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