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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

The Full-Length Individual Proviral Sequencing (FLIPS) assay is an efficient and high-throughput method designed to amplify and sequence single, near full-length (intact and defective), HIV-1 proviruses. FLIPS allows determination of the genetic composition of integrated HIV-1 within a cell populati...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Hiener, Bonnie, Eden, John-Sebastian, Horsburgh, Bethany A., Palmer, Sarah
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235535/
https://ncbi.nlm.nih.gov/pubmed/30394382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58016
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