Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Probing an individual cell's gene expression enables the identification of cell type and cell state. Single-cell RNA sequencing has emerged as a powerful tool for studying transcriptional profiles of cells, particularly in heterogeneous tissues such as the central nervous system. However, disso...

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Dettagli Bibliografici
Pubblicato in:J Vis Exp
Autori principali: Matson, Kaya J.E., Sathyamurthy, Anupama, Johnson, Kory R., Kelly, Michael C., Kelley, Matthew W., Levine, Ariel J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2018
Soggetti:
Neuroscience
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235529/
https://ncbi.nlm.nih.gov/pubmed/30371670
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58413
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