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Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples

Infection with Zika virus (ZIKV) can be asymptomatic in adults, however, infection during pregnancy can lead to miscarriage and severe neurological birth defects. The goal of this protocol is to quickly detect ZIKV in both human and mosquito samples. The current gold standard for ZIKV detection is q...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Bartolone, Sarah N., Tree, Maya O., Conway, Michael J., Chancellor, Michael B., Lamb, Laura E.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235193/
https://ncbi.nlm.nih.gov/pubmed/30272674
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58436
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