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Luminophore Formation in Various Conformations of Bovine Serum Albumin by Binding of Gold(III)
The purpose of the presented protocols is to study the process of Au(III) binding to BSA, yielding conformation change-induced red fluorescence (λ(em) = 640 nm) of BSA-Au(III) complexes. The method adjusts the pH to show that the emergence of the red fluorescence is correlated with the pH-induced eq...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6235082/ https://ncbi.nlm.nih.gov/pubmed/30222150 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58141 |
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