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Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding

In this article, we describe the method that allows fluorescently tagged structures such as axons to be targeted for electron microscopy (EM) analysis without the need to convert their labels into electron dense stains, introduce any fiducial marks, or image large volumes at high resolution. We opti...

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Detalhes bibliográficos
Publicado no:Front Neuroanat
Main Authors: Maclachlan, Catherine, Sahlender, Daniela A., Hayashi, Shuichi, Molnár, Zoltán, Knott, Graham
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6232369/
https://ncbi.nlm.nih.gov/pubmed/30459565
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fnana.2018.00088
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