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Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Alternative splicing (AS) occurs in more than 90% of human genes. The expression pattern of an alternatively spliced exon is often regulated in a cell type-specific fashion. AS expression patterns are typically analyzed by RT-PCR and RNA-seq using RNA samples isolated from a population of cells. In...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Guo, Xuan, Zhao, Yiqing, Nguyen, Hieu, Liu, Tonghua, Wang, Zhenghe, Lou, Hua
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6231910/
https://ncbi.nlm.nih.gov/pubmed/30199013
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57889
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