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A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells
Hematopoietic stem cells (HSCs) are rare cells, with the mouse bone marrow containing only ~25,000 phenotypic long term repopulating HSCs. A Western blotting protocol was optimized and suitable for the analysis of small numbers of HSCs (500 - 15,000 cells). Phenotypic HSCs were purified, accurately...
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| Published in: | J Vis Exp |
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| Main Authors: | , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
MyJove Corporation
2018
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6231698/ https://ncbi.nlm.nih.gov/pubmed/30199018 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56855 |
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