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Adapting the chemical unfolding assay for high-throughput protein screening using experimental and spectroscopic corrections
The chemical unfolding (denaturation) assay can be used to calculate the change in the Gibbs free energy of unfolding, ΔG, and inflection point of unfolding, to collectively inform on molecule stability. Here, we evaluated methods for calculating the ΔG across 23 monoclonal antibody sequence variant...
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| Publicado no: | Anal Biochem |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Elsevier
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6226613/ https://ncbi.nlm.nih.gov/pubmed/30236889 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2018.08.027 |
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