RepB C-terminus mutation of a pRi-repABC binary vector affects plasmid copy number in Agrobacterium and transgene copy number in plants

A native repABC replication origin from pRiA4b was previously reported as a single copy plasmid in Agrobacterium tumefaciens and can improve the production of transgenic plants with a single copy insertion of transgenes when it is used in binary vectors for Agrobacterium-mediated transformation. A h...

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Veröffentlicht in:PLoS One
Hauptverfasser: Vaghchhipawala, Zarir, Radke, Sharon, Nagy, Ervin, Russell, Mary L., Johnson, Susan, Gelvin, Stanton B., Gilbertson, Larry A., Ye, Xudong
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2018
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6226153/
https://ncbi.nlm.nih.gov/pubmed/30412579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0200972
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