Effects of CRISPR/Cas9 dosage on TICAM1 and RBL gene mutation rate, embryonic development, hatchability and fry survival in channel catfish

The current study was conducted to assess the effects of microinjection of different dosages of guide RNA (gRNA)/Cas9 protein on the mutation rate, embryo survival, embryonic development, hatchability and early fry survival in channel catfish, Ictalurus punctatus. Guide RNAs targeting two of the cha...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Elaswad, Ahmed, Khalil, Karim, Ye, Zhi, Liu, Zhanjiang, Liu, Shikai, Peatman, Eric, Odin, Ramjie, Vo, Khoi, Drescher, David, Gosh, Kamal, Qin, Guyu, Bugg, William, Backenstose, Nathan, Dunham, Rex
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6220201/
https://ncbi.nlm.nih.gov/pubmed/30405210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-34738-4
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