Selection and validation of suitable reference genes for qPCR gene expression analysis in goats and sheep under Peste des petits ruminants virus (PPRV), lineage IV infection

Identification of suitable candidate reference genes is an important prerequisite for validating the gene expression data obtained from downstream analysis of RNA sequencing using quantitative real time PCR (qRT-PCR). Though existence of a universal reference gene is myth, commonly used reference ge...

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Détails bibliographiques
Publié dans:Sci Rep
Auteurs principaux: Sahu, Amit Ranjan, Wani, Sajad Ahmad, Saxena, Shikha, Rajak, Kaushal Kishor, Chaudhary, Dheeraj, Sahoo, Aditya Prasad, Khanduri, Alok, Pandey, Aruna, Mondal, Piyali, Malla, Waseem Akram, Khan, Raja Ishaq Nabi, Tiwari, Ashok Kumar, Mishra, Bina, Muthuchelvan, D., Mishra, Bishnu Prasad, Singh, Raj Kumar, Gandham, Ravi Kumar
Format: Artigo
Langue:Inglês
Publié: Nature Publishing Group UK 2018
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Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6206032/
https://ncbi.nlm.nih.gov/pubmed/30374051
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-34236-7
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