Robustness of biomarker determination in breast cancer by RT-qPCR: impact of tumor cell content, DCIS and non-neoplastic breast tissue

BACKGROUND: Tissue heterogeneity in formalin-fixed paraffin-embedded (FFPE) breast cancer specimens may affect the accuracy of reverse transcription quantitative real-time PCR (RT-qPCR). Herein, we tested the impact of tissue heterogeneity of breast cancer specimen on the RT-qPCR-based gene expressi...

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Detalhes bibliográficos
Publicado no:Diagn Pathol
Main Authors: Hartmann, Kerstin, Schlombs, Kornelia, Laible, Mark, Gürtler, Claudia, Schmidt, Marcus, Sahin, Ugur, Lehr, Hans-Anton
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2018
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6195967/
https://ncbi.nlm.nih.gov/pubmed/30342538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13000-018-0760-6
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