Monomerization of the photoconvertible fluorescent protein SAASoti by rational mutagenesis of single amino acids

Photoconvertible fluorescent proteins (PCFPs) are widely used as markers for the visualization of intracellular processes and for sub-diffraction single-molecule localization microscopy. Although wild type of a new photoconvertible fluorescent protein SAASoti tends to aggregate, we succeeded, via ra...

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Published in:Sci Rep
Main Authors: Solovyev, Ilya D., Gavshina, Alexandra V., Katti, Aditya S., Chizhik, Alexey I., Vinokurov, Leonid M., Lapshin, Grigory D., Ivashina, Tatiana V., Khrenova, Maria G., Kireev, Igor I., Gregor, Ingo, Enderlein, Jörg, Savitsky, Alexander P.
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2018
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6195611/
https://ncbi.nlm.nih.gov/pubmed/30341334
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-33250-z
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