Fast and inexpensive protocols for consistent extraction of high quality DNA and RNA from challenging plant and fungal samples for high-throughput SNP genotyping and sequencing applications

Modern genotyping techniques, such as SNP analysis and genotyping by sequencing (GBS), are hampered by poor DNA quality and purity, particularly in challenging plant species, rich in secondary metabolites. We therefore investigated the utility of a pre-wash step using a buffered sorbitol solution, p...

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Detaylı Bibliyografya
Yayımlandı:PLoS One
Asıl Yazarlar: Inglis, Peter W., Pappas, Marilia de Castro R., Resende, Lucileide V., Grattapaglia, Dario
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Public Library of Science 2018
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC6193717/
https://ncbi.nlm.nih.gov/pubmed/30335843
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0206085
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