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Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing

Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 s...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Genomics Inform
Hauptverfasser: Lee, Minho, Choi, Shin-Jung, Han, Sangjo, Nam, Miyoung, Kim, Dongsup, Kim, Dong-Uk, Hoe, Kwang-Lae
Format: Artigo
Sprache:Inglês
Veröffentlicht: Korea Genome Organization 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6187811/
https://ncbi.nlm.nih.gov/pubmed/30304922
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5808/GI.2018.16.2.22
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