Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing

Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 s...

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Publicat a:Genomics Inform
Autors principals: Lee, Minho, Choi, Shin-Jung, Han, Sangjo, Nam, Miyoung, Kim, Dongsup, Kim, Dong-Uk, Hoe, Kwang-Lae
Format: Artigo
Idioma:Inglês
Publicat: Korea Genome Organization 2018
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Original Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6187811/
https://ncbi.nlm.nih.gov/pubmed/30304922
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5808/GI.2018.16.2.22
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