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Plate Design for and Cherry Picking of Bacterial RNAi Clones for Systematic Error Detection in High-Throughput C. elegans RNAi Screens

Automated or semi-automated high-throughput RNAi screens are highly prone to systematic errors because of multistep repetitive protocols and extensive use of automated instruments. A well-designed RNAi library can help detect and minimize systematic human and robotic errors. In this Unit, we describ...

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Detalhes bibliográficos
Publicado no:Curr Protoc Mol Biol
Main Authors: Jagadeesan, Sakthimala, Hakkim, Abdul
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6168385/
https://ncbi.nlm.nih.gov/pubmed/30192421
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpmb.70
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