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Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells

[Image: see text] Förster resonance energy transfer (FRET)-based sensors are a valuable tool to quantify cell biology, yet it remains necessary to identify and prevent potential artifacts in order to exploit their full potential. We show here that artifacts arising from slow donor mCerulean3 maturat...

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Detalhes bibliográficos
Publicado no:ACS Sens
Main Authors: Liu, Boqun, Mavrova, Sara N., van den Berg, Jonas, Kristensen, Sebastian K., Mantovanelli, Luca, Veenhoff, Liesbeth M., Poolman, Bert, Boersma, Arnold J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2018
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6167724/
https://ncbi.nlm.nih.gov/pubmed/30168711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssensors.8b00473
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