Influence of Fluorescent Protein Maturation on FRET Measurements in Living Cells

[Image: see text] Förster resonance energy transfer (FRET)-based sensors are a valuable tool to quantify cell biology, yet it remains necessary to identify and prevent potential artifacts in order to exploit their full potential. We show here that artifacts arising from slow donor mCerulean3 maturat...

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Pubblicato in:ACS Sens
Autori principali: Liu, Boqun, Mavrova, Sara N., van den Berg, Jonas, Kristensen, Sebastian K., Mantovanelli, Luca, Veenhoff, Liesbeth M., Poolman, Bert, Boersma, Arnold J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Chemical Society 2018
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6167724/
https://ncbi.nlm.nih.gov/pubmed/30168711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssensors.8b00473
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