Triplex Real-Time PCR without DNA Extraction for the Monitoring of Meningococcal Disease

Detection of Neisseria meningitidis has become less time- and resource-intensive with a monoplex direct real-time PCR (drt-PCR) to amplify genes from clinical specimens without DNA extraction. To further improve efficiency, we evaluated two triplex drt-PCR assays for the detection of meningococcal s...

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Publicado en:Diagnostics (Basel)
Autores principales: Whaley, Melissa J., Jenkins, Laurel T., Hu, Fang, Chen, Alexander, Diarra, Seydou, Ouédraogo-Traoré, Rasmata, Sacchi, Claudio T., Wang, Xin
Formato: Artigo
Lenguaje:Inglês
Publicado: MDPI 2018
Materias:
Communication
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6163423/
https://ncbi.nlm.nih.gov/pubmed/30200184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/diagnostics8030058
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