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Generation of Ribozymes by Rolling Circle Transcription of Promoterless Single-Stranded DNA Circles in Mammalian Cells
Self-processing hairpin ribozymes have been synthesized from promoterless single-stranded DNA circles (73 nt) within mammalian cells. Following lipid-mediated transient transfection, DNA circles were efficiently internalized by mouse L cells (OST7–1) that stably express T7 RNA polymerase confining i...
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| Published in: | Turk Biyokim Derg |
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| Main Author: | |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2006
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6159908/ https://ncbi.nlm.nih.gov/pubmed/30271230 |
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