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Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat

The current application of genome editing to crop plants is limited to cultivars that are amenable to in vitro culture and regeneration. Here, we report an in planta genome-editing which does not require callus culture and regeneration. Shoot apical meristems (SAMs) contain a subepidermal cell layer...

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Bibliografiske detaljer
Udgivet i:Sci Rep
Main Authors: Hamada, Haruyasu, Liu, Yuelin, Nagira, Yozo, Miki, Ryuji, Taoka, Naoaki, Imai, Ryozo
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6158239/
https://ncbi.nlm.nih.gov/pubmed/30258105
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-32714-6
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