Machining protein microcrystals for structure determination by electron diffraction

We demonstrate that ion-beam milling of frozen, hydrated protein crystals to thin lamella preserves the crystal lattice to near-atomic resolution. This provides a vehicle for protein structure determination, bridging the crystal size gap between the nanometer scale of conventional electron diffracti...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Autores principales: Duyvesteyn, Helen M. E., Kotecha, Abhay, Ginn, Helen M., Hecksel, Corey W., Beale, Emma V., de Haas, Felix, Evans, Gwyndaf, Zhang, Peijun, Chiu, Wah, Stuart, David I.
Formato: Artigo
Lenguaje:Inglês
Publicado: National Academy of Sciences 2018
Materias:
Biological Sciences
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC6156647/
https://ncbi.nlm.nih.gov/pubmed/30171169
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1809978115
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares