Detecting and Discriminating Shigella sonnei Using an Aptamer-Based Fluorescent Biosensor Platform

In this paper, a Whole-Bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Shigella sonnei. Real-time PCR amplification and post-SELEX experiment revealed that the selected aptmers possessed a high binding affinity and specificity for S. sonnei. Of the 21 aptamers tes...

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Vydáno v:Molecules
Hlavní autoři: Song, Myeong-Sub, Sekhon, Simranjeet Singh, Shin, Woo-Ri, Kim, Hyung Cheol, Min, Jiho, Ahn, Ji-Young, Kim, Yang-Hoon
Médium: Artigo
Jazyk:Inglês
Vydáno: MDPI 2017
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6154610/
https://ncbi.nlm.nih.gov/pubmed/28513559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules22050825
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