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Overexpression of Outer Membrane Porins in E. coli Using pBluescript-Derived Vectors

The genes coding for four major outer membrane porins of Escherichia coli, ompF, ompC, phoE, and lamB, have been cloned into pBluescript-derived vectors and overexpressed to very high level (approximately 80% of the total membrane protein) in widely used host strains lacking one or more porins. For...

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Publicat a:Gene Expr
Autors principals: Ghosh, Robin, Steiert, Matthias, Hardmeyer, Ariane, Wang, Yan-Fei, Rosenbusch, Jurg P.
Format: Artigo
Idioma:Inglês
Publicat: Cognizant Communication Corporation 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6151949/
https://ncbi.nlm.nih.gov/pubmed/9840808
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