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Improving the Catalytic Property of the Glycoside Hydrolase LXYL-P1–2 by Directed Evolution

The glycoside hydrolase LXYL-P1–2 from Lentinula edodes can specifically hydrolyze 7-β-xylosyltaxanes to form 7-β-hydroxyltaxanes for the semi-synthesis of paclitaxel. In order to improve the catalytic properties of the enzyme, we performed error-prone PCR to construct the random mutant library of L...

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Detalhes bibliográficos
Publicado no:Molecules
Main Authors: Chen, Jing-Jing, Liang, Xiao, Li, Hui-Xian, Chen, Tian-Jiao, Zhu, Ping
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6149855/
https://ncbi.nlm.nih.gov/pubmed/29207529
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules22122133
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