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Improving the Catalytic Property of the Glycoside Hydrolase LXYL-P1–2 by Directed Evolution
The glycoside hydrolase LXYL-P1–2 from Lentinula edodes can specifically hydrolyze 7-β-xylosyltaxanes to form 7-β-hydroxyltaxanes for the semi-synthesis of paclitaxel. In order to improve the catalytic properties of the enzyme, we performed error-prone PCR to construct the random mutant library of L...
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| Pubblicato in: | Molecules |
|---|---|
| Autori principali: | , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
MDPI
2017
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6149855/ https://ncbi.nlm.nih.gov/pubmed/29207529 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules22122133 |
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