Simultaneously Measuring Image Features and Resolution in Live-Cell STED Images

Reliable interpretation and quantification of cellular features in fluorescence microscopy requires an accurate estimate of microscope resolution. This is typically obtained by measuring the image of a nonbiological proxy for a point-like object, such as a fluorescent bead. Although appropriate for...

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Bibliographic Details
Published in:Biophys J
Main Authors: Barentine, Andrew E.S., Schroeder, Lena K., Graff, Michael, Baddeley, David, Bewersdorf, Joerg
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2018
Subjects:
Computational Tool
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6139878/
https://ncbi.nlm.nih.gov/pubmed/30139523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2018.07.028
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