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Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking

Super-resolution imaging and single-particle tracking require cells to be immobile as any movement reduces the resolution of the measurements. Here, we present a method based on APTES-glutaraldehyde coating of glass surfaces to immobilize cells without compromising their growth. Our method of immobi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Sci Rep
Hauptverfasser: Syga, Łukasz, Spakman, Dian, Punter, Christiaan M., Poolman, Bert
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6137044/
https://ncbi.nlm.nih.gov/pubmed/30213985
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-32166-y
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