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Super-silent FRET Sensor Enables Live Cell Imaging and Flow Cytometric Stratification of Intracellular Serine Protease Activity in Neutrophils

Serine proteases are released by neutrophils to act primarily as antimicrobial proteins but excessive and unbalanced serine protease activity results in serious host tissue damage. Here the synthesis of a novel chemical sensor based on a multi-branched fluorescence quencher is reported. It is super-...

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Dettagli Bibliografici
Pubblicato in:Sci Rep
Autori principali: Craven, Thomas H., Avlonitis, Nicolaos, McDonald, Neil, Walton, Tashfeen, Scholefield, Emma, Akram, Ahsan R., Walsh, Timothy S., Haslett, Chris, Bradley, Mark, Dhaliwal, Kevin
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6131393/
https://ncbi.nlm.nih.gov/pubmed/30201982
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-31391-9
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