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Reconstitution and substrate specificity for isopentenyl pyrophosphate of the antiviral radical SAM enzyme viperin

Viperin is a radical SAM enzyme that has been shown to possess antiviral activity against a broad spectrum of viruses; however, its molecular mechanism is unknown. We report here that recombinant fungal and archaeal viperin enzymes catalyze the addition of the 5′-deoxyadenosyl radical (5′-dA(•)) to...

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Veröffentlicht in:J Biol Chem
Hauptverfasser: Chakravarti, Arpita, Selvadurai, Kiruthika, Shahoei, Rezvan, Lee, Hugo, Fatma, Shirin, Tajkhorshid, Emad, Huang, Raven H.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Biochemistry and Molecular Biology 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6130951/
https://ncbi.nlm.nih.gov/pubmed/30030381
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA118.003998
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