Optimized base editors enable efficient editing in cells, organoids and mice

CRISPR base editing enables the creation of targeted single-base conversions without generating double stranded breaks. However, the efficiency of current base editors is very low in many cell types. We re-engineered the sequences of BE3, BE4Gam, and xBE3 by codon optimization and incorporation of a...

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Vydáno v:Nat Biotechnol
Hlavní autoři: Zafra, Maria Paz, Schatoff, Emma M, Katti, Alyna, Foronda, Miguel, Breinig, Marco, Schweitzer, Anabel Y., Simon, Amber, Han, Teng, Goswami, Sukanya, Montgomery, Emma, Thibado, Jordana, Kastenhuber, Edward R, Sánchez-Rivera, Francisco J., Shi, Junwei, Vakoc, Christopher R, Lowe, Scott W, Tschaharganeh, Darjus F., Dow, Lukas E
Médium: Artigo
Jazyk:Inglês
Vydáno: 2018
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6130889/
https://ncbi.nlm.nih.gov/pubmed/29969439
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.4194
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