An enhanced CRISPR repressor for targeted mammalian gene regulation

The RNA-guided endonuclease Cas9 can be converted into a programmable transcriptional repressor, yet inefficiencies in target gene silencing have limited its utility. Here we describe an improved Cas9 repressor based on the C-terminal fusion of a rationally designed bipartite repressor domain, KRAB-...

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Veröffentlicht in:Nat Methods
Hauptverfasser: Yeo, Nan Cher, Chavez, Alejandro, Lance-Byrne, Alissa, Chan, Yingleong, Menn, David, Milanova, Denitsa, Kuo, Chih-Chung, Guo, Xiaoge, Sharma, Sumana, Tung, Angela, Cecchi, Ryan J., Tuttle, Marcelle, Pradhan, Swechchha, Lim, Elaine T, Davidsohn, Noah, Ebrahimkhani, Mo R., Collins, James J., Lewis, Nathan E., Kiani, Samira, Church, George M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6129399/
https://ncbi.nlm.nih.gov/pubmed/30013045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-018-0048-5
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