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Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

The presence of ribonucleotides in nuclear DNA has been shown to be a source of genomic instability. The extent of ribonucleotide incorporation can be assessed by alkaline hydrolysis and gel electrophoresis as RNA is highly susceptible to hydrolysis in alkaline conditions. This, in combination with...

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Publicat a:J Vis Exp
Autors principals: Zhou, Zhi-Xiong, Williams, Jessica S., Kunkel, Thomas A.
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2018
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6126554/
https://ncbi.nlm.nih.gov/pubmed/30102287
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58020
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