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The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

From behavior to gene expression, circadian rhythms regulate nearly all aspects of physiology. Here, we present a methodology to challenge mouse splenocytes with the pathogen-associated molecular patterns (PAMPs) lipopolysaccharide (LPS), ODN1826, and heat-killed Listeria monocytogenes and examine t...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Hovedforfatter: Silver, Adam C.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6126544/
https://ncbi.nlm.nih.gov/pubmed/30102285
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58022
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