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Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion
The ability to precisely edit individual bases of bacterial genomes would accelerate the investigation of the function of genes. Here we utilized a nickase Cas9-cytidine deaminase fusion protein to direct the conversion of cytosine to thymine within prokaryotic cells, resulting in high mutagenesis f...
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| Publicado no: | Commun Biol |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6123677/ https://ncbi.nlm.nih.gov/pubmed/30271918 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-018-0035-5 |
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